Hotspot

rps9

Genome · 105230-105682 · 453 bp

Back to Porosira

Pi 0.13

MarkerSeek score 80.40

Primer available yes

Label rps9

Feature Metadata

label_name: rps9
feature_type: gene
region: Genome
length_bp: 453
variable_sites: 55
pi: 0.13
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps9_p1	GGCCGTAAATCAGCAGATGA	ATCTTTTACTCGGCCACCAC	50.0	50.0	58.0	57.9	1501-1532 bp	1.0	88.4
rps9_p2	GGCCGTAAATCAGCAGATGA	TGTACAAACACCACGTCGTT	50.0	45.0	58.0	58.0	1370 bp	0.5	59.6
rps9_p3	GGCCGTAAATCAGCAGATGA	CTCGGCCACCACGTATTAAA	50.0	50.0	58.0	58.0	1524 bp	0.5	59.5
rps9_p4	GGCCGTAAATCAGCAGATGA	ACCAAAGCAGGTGATTGTGT	50.0	45.0	58.0	57.9	1339 bp	0.5	59.1
rps9_p5	GGCCGTAAATCAGCAGATGA	CGAACTCGAGCAACTTTTCG	50.0	50.0	58.0	57.8	1432 bp	0.5	58.0