Hotspot

dnaK

Genome · 90685-92493 · 1809 bp

Back to Porosira

Pi 0.213

MarkerSeek score 79.00

Primer available yes

Label dnaK

Feature Metadata

label_name: dnaK
feature_type: gene
region: Genome
length_bp: 1809
variable_sites: 382
pi: 0.213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
dnaK_p1	GGAGGAGCAACATTAGCACA	GATCTTTCTGGAACCGTCCC	50.0	55.0	57.9	58.0	2721 bp	0.5	58.9
dnaK_p2	GGAGGAGCAACATTAGCACA	AATACATTGCCTCAGCCTGG	50.0	50.0	57.9	57.9	2359 bp	0.5	58.6
dnaK_p3	GGAGGAGCAACATTAGCACA	CATTGCCTCAGCCTGGTTAT	50.0	50.0	57.9	57.9	2355 bp	0.5	58.6
dnaK_p4	GGAGGAGCAACATTAGCACA	ATGTGTCATTGGACCTCGTG	50.0	50.0	57.9	57.9	2950 bp	0.5	58.3
dnaK_p5	GGAGGAGCAACATTAGCACA	CATGTGTCATTGGACCTCGT	50.0	50.0	57.9	57.9	2951 bp	0.5	58.3