Hotspot

clpC

Genome · 109098-111803 · 2706 bp

Back to Porosira

Pi 0.232

MarkerSeek score 78.00

Primer available yes

Label clpC

Feature Metadata

label_name: clpC
feature_type: gene
region: Genome
length_bp: 2706
variable_sites: 621
pi: 0.232
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpC_p1	GGGGATCGAGGATTTGCAAT	GACTTCGTCGTTCCAGATCA	50.0	50.0	58.0	57.4	2996 bp	0.5	54.8
clpC_p2	CCTTACCACTTGGCCATACC	GACTTCGTCGTTCCAGATCA	55.0	50.0	57.9	57.4	2970 bp	0.5	54.5
clpC_p3	TTGCAATCCACTGCCTTACC	GACTTCGTCGTTCCAGATCA	50.0	50.0	58.5	57.4	2983 bp	0.5	51.3
clpC_p4	ATCCACTGCCTTACCACTTG	GACTTCGTCGTTCCAGATCA	50.0	50.0	57.5	57.4	2978 bp	0.5	51.0
clpC_p5	CAATCCACTGCCTTACCACT	GACTTCGTCGTTCCAGATCA	50.0	50.0	57.5	57.4	2980 bp	0.5	51.0