Hotspot

trnI-GAU-rns

Genome · 128291-128346 · 56 bp

Back to Porosira

Pi 0.125
MarkerSeek score 84.00
Primer available yes
Label trnI-GAU-rns

Feature Metadata

label_name
trnI-GAU-rns
feature_type
igs
region
Genome
length_bp
56
variable_sites
7
pi
0.125
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-GAU-rns_p1 CTGGGCCATTCTGGATTTGA GACTGGGGTGAAGTCGTAAC 50.0 55.0 57.9 57.9 192 bp 1.0 81.1
trnI-GAU-rns_p2 CTGGGCCATTCTGGATTTGA GGAGGGTGCCTAAGGTAGAA 50.0 55.0 57.9 58.1 217 bp 1.0 80.4
trnI-GAU-rns_p3 CTGGGCCATTCTGGATTTGA GGGGTGAAGTCGTAACAAGG 50.0 55.0 57.9 58.2 188 bp 1.0 80.2
trnI-GAU-rns_p4 CTGGGCCATTCTGGATTTGA GTAACCGTACTGGAAGGTGC 50.0 55.0 57.9 58.3 169 bp 1.0 79.9
trnI-GAU-rns_p5 CTGGGCCATTCTGGATTTGA TGACTGGGGTGAAGTCGTAA 50.0 50.0 57.9 58.3 193 bp 1.0 79.4