Hotspot

trnR-UCU-psaE

Genome · 22864-23041 · 178 bp

Back to Porosira

Pi 0.339

MarkerSeek score 81.00

Primer available yes

Label trnR-UCU-psaE

Feature Metadata

label_name: trnR-UCU-psaE
feature_type: igs
region: Genome
length_bp: 178
variable_sites: 56
pi: 0.339
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-psaE_p1	TTCGATTCCTACTGGGCCTA	ACGAACTTTTGAATTACGATCA	50.0	31.8	57.5	54.5	216-225 bp	1.0	52.0
trnR-UCU-psaE_p2	AACGGATAGGACAGGAACCT	ACGAACTTTTGAATTACGATCA	50.0	31.8	57.8	54.5	264 bp	0.5	20.0
trnR-UCU-psaE_p3	ACGGATAGGACAGGAACCTT	ACGAACTTTTGAATTACGATCA	50.0	31.8	57.8	54.5	263 bp	0.5	20.0
trnR-UCU-psaE_p4	AACGGATAGGACAGGAACCT	ACGAACTTTTGAATTACGATCAA	50.0	30.4	57.8	55.2	264 bp	0.5	20.0
trnR-UCU-psaE_p5	ACGGATAGGACAGGAACCTT	ACGAACTTTTGAATTACGATCAA	50.0	30.4	57.8	55.2	263 bp	0.5	20.0