primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
sufC_p1	sufC	1	AAATGAGAAAGGTCAGGGCG	GCCTCCGCTAAGAATGTGAA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.197791	14840	15601	1563-1563	0.500000	58.4
sufC_p2	sufC	2	AAATGAGAAAGGTCAGGGCG	TGCAAACTCTCCCTCTGGTA	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.197791	14840	15601	1891-1891	0.500000	58.4
sufC_p3	sufC	3	AAATGAGAAAGGTCAGGGCG	GAATCCGATCAGCTTCACGT	20	20	50.0	50.0	58.2	58.1	0.0	0.0	0.245720	14840	15601	1697-1697	0.500000	58.0
sufC_p4	sufC	4	AAATGAGAAAGGTCAGGGCG	ATGCACTAAGAAACCGGCAA	20	20	50.0	45.0	58.2	58.1	0.0	0.0	0.269328	14840	15601	1612-1612	0.500000	57.8
sufC_p5	sufC	5	AAATGAGAAAGGTCAGGGCG	TGCACTAAGAAACCGGCAAT	20	20	50.0	45.0	58.2	58.1	0.0	0.0	0.269328	14840	15601	1611-1611	0.500000	57.8
trnR-UCU-psaE_p1	trnR-UCU-psaE	1	TTCGATTCCTACTGGGCCTA	ACGAACTTTTGAATTACGATCA	20	22	50.0	31.8	57.5	54.5	4.5	0.0	6.012123	22864	23041	216-225	1.000000	52.0
trnR-UCU-psaE_p2	trnR-UCU-psaE	2	AACGGATAGGACAGGAACCT	ACGAACTTTTGAATTACGATCA	20	22	50.0	31.8	57.8	54.5	0.0	0.0	5.796637	22864	23041	264-264	0.500000	20.0
trnR-UCU-psaE_p3	trnR-UCU-psaE	3	ACGGATAGGACAGGAACCTT	ACGAACTTTTGAATTACGATCA	20	22	50.0	31.8	57.8	54.5	0.0	0.0	5.796637	22864	23041	263-263	0.500000	20.0
trnR-UCU-psaE_p4	trnR-UCU-psaE	4	AACGGATAGGACAGGAACCT	ACGAACTTTTGAATTACGATCAA	20	23	50.0	30.4	57.8	55.2	0.0	0.0	6.034582	22864	23041	264-264	0.500000	20.0
trnR-UCU-psaE_p5	trnR-UCU-psaE	5	ACGGATAGGACAGGAACCTT	ACGAACTTTTGAATTACGATCAA	20	23	50.0	30.4	57.8	55.2	0.0	0.0	6.034582	22864	23041	263-263	0.500000	20.0
rps2_p1	rps2	1	AACAAAATCGCCCTTCTGGT	CACCATTACTACCACGCGAA	20	20	45.0	50.0	58.0	58.0	0.0	15.9	0.005575	29910	30635	1797-1797	0.500000	60.0
rps2_p2	rps2	2	AACAAAATCGCCCTTCTGGT	ACCCAAGCTTTGAATTCCGT	20	20	45.0	45.0	58.0	58.0	0.0	2.3	0.009247	29910	30635	2118-2118	0.500000	59.9
rps2_p3	rps2	3	CTCCAGTTGCAGCTGAAGAA	CACCATTACTACCACGCGAA	20	20	50.0	50.0	58.1	58.0	29.7	15.9	0.112737	29910	30635	1497-1497	0.500000	59.1
rps2_p4	rps2	4	TCCAGTTGCAGCTGAAGAAG	CACCATTACTACCACGCGAA	20	20	50.0	50.0	58.1	58.0	29.7	15.9	0.112737	29910	30635	1496-1496	0.500000	59.1
rps2_p5	rps2	5	TCCAGTTGCAGCTGAAGAAG	ACCCAAGCTTTGAATTCCGT	20	20	50.0	45.0	58.1	58.0	29.7	2.3	0.116409	29910	30635	1817-1817	0.500000	59.1
rpoB-rps20_p1	rpoB-rps20	1	TCACTGGGAATTACGCGTTT	CTGCTCAACTAAAAGCTGCG	20	20	45.0	50.0	57.8	57.8	20.8	0.0	0.411096	41472	42493	1491-1491	0.500000	56.7
rpoB-rps20_p2	rpoB-rps20	2	TTCACTGGGAATTACGCGTT	CTGCTCAACTAAAAGCTGCG	20	20	45.0	50.0	57.8	57.8	20.8	0.0	0.411096	41472	42493	1492-1492	0.500000	56.7
rpoB-rps20_p3	rpoB-rps20	3	TTTCACTGGGAATTACGCGT	CTGCTCAACTAAAAGCTGCG	20	20	45.0	50.0	57.8	57.8	19.2	0.0	0.411096	41472	42493	1493-1493	0.500000	56.7
rpoB-rps20_p4	rpoB-rps20	4	GGTTGTCCATCCCAATTCCA	CTGCTCAACTAAAAGCTGCG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.468375	41472	42493	1653-1653	0.500000	56.3
rpoB-rps20_p5	rpoB-rps20	5	TGGTTGTCCATCCCAATTCC	CTGCTCAACTAAAAGCTGCG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.468375	41472	42493	1654-1654	0.500000	56.3
trnT-UGU-ycf33_p1	trnT-UGU-ycf33	1	TGGTGTAGCTCAACGGTAGA	CGACCTTGGGCTTATGAGTC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.144897	52287	52563	707-735	1.000000	90.8
trnT-UGU-ycf33_p2	trnT-UGU-ycf33	2	GATTCCGGGTTTTCAGGGTT	CGACCTTGGGCTTATGAGTC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.139435	52287	52563	1154-1154	0.500000	58.9
trnT-UGU-ycf33_p3	trnT-UGU-ycf33	3	TTGATTCCGGGTTTTCAGGG	CGACCTTGGGCTTATGAGTC	20	20	50.0	55.0	58.1	58.1	0.0	0.0	0.140803	52287	52563	1156-1156	0.500000	58.9
trnT-UGU-ycf33_p4	trnT-UGU-ycf33	4	GGCGATTCCCTAATTCTGGG	CGACCTTGGGCTTATGAGTC	20	20	55.0	55.0	58.1	58.1	9.5	0.0	0.161115	52287	52563	1318-1318	0.500000	58.7
trnT-UGU-ycf33_p5	trnT-UGU-ycf33	5	GATTCCGGGTTTTCAGGGTT	TAGTAGATGGCTCTGGTGGG	20	20	50.0	55.0	58.1	57.9	0.0	0.0	0.168864	52287	52563	1185-1185	0.500000	58.6
rns-trnI-GAU_p1	rns-trnI-GAU	1	GACTGGGGTGAAGTCGTAAC	CTGGGCCATTCTGGATTTGA	20	20	55.0	50.0	57.9	57.9	0.0	6.7	0.224425	71711	71766	192-192	1.000000	81.1
rns-trnI-GAU_p2	rns-trnI-GAU	2	GGAGGGTGCCTAAGGTAGAA	CTGGGCCATTCTGGATTTGA	20	20	55.0	50.0	58.1	57.9	0.0	6.7	0.272957	71711	71766	217-217	1.000000	80.4
rns-trnI-GAU_p3	rns-trnI-GAU	3	GGGGTGAAGTCGTAACAAGG	CTGGGCCATTCTGGATTTGA	20	20	55.0	50.0	58.2	57.9	0.0	6.7	0.343845	71711	71766	188-188	1.000000	80.2
rns-trnI-GAU_p4	rns-trnI-GAU	4	GTAACCGTACTGGAAGGTGC	CTGGGCCATTCTGGATTTGA	20	20	55.0	50.0	58.3	57.9	0.0	6.7	0.430050	71711	71766	169-169	1.000000	79.9
rns-trnI-GAU_p5	rns-trnI-GAU	5	TGACTGGGGTGAAGTCGTAA	CTGGGCCATTCTGGATTTGA	20	20	50.0	50.0	58.3	57.9	0.0	6.7	0.441339	71711	71766	193-193	1.000000	79.4
rps16_p1	rps16	1	TGCAAAGCTGGTACTAATGGA	CGTCGTGAAGGAGTAACTGG	21	20	42.9	55.0	57.3	58.0	0.0	0.0	1.733474	86805	87038	1394-1394	0.500000	46.1
rps16_p2	rps16	2	TGCAAAGCTGGTACTAATGGA	CCATGGCCACGTTACTGTAA	21	20	42.9	50.0	57.3	57.9	0.0	8.2	1.814086	86805	87038	1278-1278	0.500000	45.5
rps16_p3	rps16	3	TGCAAAGCTGGTACTAATGGA	CGGTATCGCGGACCTAAATT	21	20	42.9	50.0	57.3	57.9	0.0	23.1	1.856498	86805	87038	1616-1616	0.500000	45.1
rps16_p4	rps16	4	TGCAAAGCTGGTACTAATGGA	TTTATGTCACGGTATCGCGG	21	20	42.9	50.0	57.3	58.2	0.0	6.1	1.869296	86805	87038	1625-1625	0.500000	45.0
rps16_p5	rps16	5	TGCAAAGCTGGTACTAATGGA	CCGGTCACTCGCCCTATATA	21	20	42.9	55.0	57.3	58.2	0.0	0.0	1.881737	86805	87038	1689-1689	0.500000	44.9
dnaK_p1	dnaK	1	GGAGGAGCAACATTAGCACA	GATCTTTCTGGAACCGTCCC	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.139449	90685	92493	2721-2721	0.500000	58.9
dnaK_p2	dnaK	2	GGAGGAGCAACATTAGCACA	AATACATTGCCTCAGCCTGG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.176831	90685	92493	2359-2359	0.500000	58.6
dnaK_p3	dnaK	3	GGAGGAGCAACATTAGCACA	CATTGCCTCAGCCTGGTTAT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.176831	90685	92493	2355-2355	0.500000	58.6
dnaK_p4	dnaK	4	GGAGGAGCAACATTAGCACA	ATGTGTCATTGGACCTCGTG	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.213002	90685	92493	2950-2950	0.500000	58.3
dnaK_p5	dnaK	5	GGAGGAGCAACATTAGCACA	CATGTGTCATTGGACCTCGT	20	20	50.0	50.0	57.9	57.9	0.0	0.0	0.213002	90685	92493	2951-2951	0.500000	58.3
rpl3_p1	rpl3	1	ATTAATCACGGCTTGACGCT	AACACCACCACCTCTCCATA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.063039	92781	93437	1454-1454	0.500000	59.5
rpl3_p2	rpl3	2	ATTAATCACGGCTTGACGCT	ACACCACCACCTCTCCATAA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.063039	92781	93437	1453-1453	0.500000	59.5
rpl3_p3	rpl3	3	ATTAATCACGGCTTGACGCT	ATAACACCACCACCTCTCCA	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.063039	92781	93437	1456-1456	0.500000	59.5
rpl3_p4	rpl3	4	ATTAATCACGGCTTGACGCT	TAACACCACCACCTCTCCAT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.063039	92781	93437	1455-1455	0.500000	59.5
rpl3_p5	rpl3	5	TCCGTAAAACTTGTGCGGAA	ACACCACCACCTCTCCATAA	20	20	45.0	50.0	58.0	58.0	8.6	0.0	0.088035	92781	93437	1634-1634	0.500000	59.3
rpl4_p1	rpl4	1	GAAACTTCGTTTGCTGTCGG	CGAATGAACAGTAGCGGGAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.023300	93486	94133	1597-1597	0.500000	59.8
rpl4_p2	rpl4	2	GGGACGGTTCCAGAAAGATC	CGAATGAACAGTAGCGGGAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.030171	93486	94133	1735-1735	0.500000	59.8
rpl4_p3	rpl4	3	CCAGGTTCGATTGGTATGGG	CGAATGAACAGTAGCGGGAA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.037264	93486	94133	1465-1465	0.500000	59.7
rpl4_p4	rpl4	4	GAAACTTCGTTTGCTGTCGG	TGATACTCCTGGCCCTGAAA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.053303	93486	94133	1726-1726	0.500000	59.6
rpl4_p5	rpl4	5	ACTCCAAAAGCGTTGGAACT	CGAATGAACAGTAGCGGGAA	20	20	45.0	50.0	57.9	58.0	21.0	0.0	0.057365	93486	94133	1529-1529	0.500000	59.5
rps9_p1	rps9	1	GGCCGTAAATCAGCAGATGA	ATCTTTTACTCGGCCACCAC	20	20	50.0	50.0	58.0	57.9	0.0	2.2	0.155866	105230	105682	1501-1532	1.000000	88.4
rps9_p2	rps9	2	GGCCGTAAATCAGCAGATGA	TGTACAAACACCACGTCGTT	20	20	50.0	45.0	58.0	58.0	0.0	0.0	0.053801	105230	105682	1370-1370	0.500000	59.6
rps9_p3	rps9	3	GGCCGTAAATCAGCAGATGA	CTCGGCCACCACGTATTAAA	20	20	50.0	50.0	58.0	58.0	0.0	2.2	0.059378	105230	105682	1524-1524	0.500000	59.5
rps9_p4	rps9	4	GGCCGTAAATCAGCAGATGA	ACCAAAGCAGGTGATTGTGT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.118219	105230	105682	1339-1339	0.500000	59.1
rps9_p5	rps9	5	GGCCGTAAATCAGCAGATGA	CGAACTCGAGCAACTTTTCG	20	20	50.0	50.0	58.0	57.8	0.0	13.6	0.244714	105230	105682	1432-1432	0.500000	58.0
clpC_p1	clpC	1	GGGGATCGAGGATTTGCAAT	GACTTCGTCGTTCCAGATCA	20	20	50.0	50.0	58.0	57.4	0.0	0.0	0.644536	109098	111803	2996-2996	0.500000	54.8
clpC_p2	clpC	2	CCTTACCACTTGGCCATACC	GACTTCGTCGTTCCAGATCA	20	20	55.0	50.0	57.9	57.4	6.1	0.0	0.684200	109098	111803	2970-2970	0.500000	54.5
clpC_p3	clpC	3	TTGCAATCCACTGCCTTACC	GACTTCGTCGTTCCAGATCA	20	20	50.0	50.0	58.5	57.4	0.0	0.0	1.086694	109098	111803	2983-2983	0.500000	51.3
clpC_p4	clpC	4	ATCCACTGCCTTACCACTTG	GACTTCGTCGTTCCAGATCA	20	20	50.0	50.0	57.5	57.4	0.0	0.0	1.128317	109098	111803	2978-2978	0.500000	51.0
clpC_p5	clpC	5	CAATCCACTGCCTTACCACT	GACTTCGTCGTTCCAGATCA	20	20	50.0	50.0	57.5	57.4	0.0	0.0	1.128317	109098	111803	2980-2980	0.500000	51.0
thiG_p1	thiG	1	AGGAGAAGGTTCAGGGTGAA	TGGTCGTAGGTTCAAGTCCT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.123774	111960	112766	1515-1515	0.500000	59.0
thiG_p2	thiG	2	ACAGAAACAACGCTCGGAAT	TGGTCGTAGGTTCAAGTCCT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.126417	111960	112766	1064-1064	0.500000	59.0
thiG_p3	thiG	3	TCAGTATTACCACCTGCCGA	TGGTCGTAGGTTCAAGTCCT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.150075	111960	112766	1579-1579	0.500000	58.8
thiG_p4	thiG	4	AGGAGAAGGTTCAGGGTGAA	TTCAAGTCCTACCTGGGGAG	20	20	50.0	55.0	57.9	58.0	0.0	0.0	0.165782	111960	112766	1505-1505	0.500000	58.7
thiG_p5	thiG	5	ACAGAAACAACGCTCGGAAT	TTCAAGTCCTACCTGGGGAG	20	20	45.0	55.0	58.1	58.0	0.0	0.0	0.168426	111960	112766	1054-1054	0.500000	58.7
trnI-GAU-rns_p1	trnI-GAU-rns	1	CTGGGCCATTCTGGATTTGA	GACTGGGGTGAAGTCGTAAC	20	20	50.0	55.0	57.9	57.9	6.7	0.0	0.224425	128291	128346	192-192	1.000000	81.1
trnI-GAU-rns_p2	trnI-GAU-rns	2	CTGGGCCATTCTGGATTTGA	GGAGGGTGCCTAAGGTAGAA	20	20	50.0	55.0	57.9	58.1	6.7	0.0	0.272957	128291	128346	217-217	1.000000	80.4
trnI-GAU-rns_p3	trnI-GAU-rns	3	CTGGGCCATTCTGGATTTGA	GGGGTGAAGTCGTAACAAGG	20	20	50.0	55.0	57.9	58.2	6.7	0.0	0.343845	128291	128346	188-188	1.000000	80.2
trnI-GAU-rns_p4	trnI-GAU-rns	4	CTGGGCCATTCTGGATTTGA	GTAACCGTACTGGAAGGTGC	20	20	50.0	55.0	57.9	58.3	6.7	0.0	0.430050	128291	128346	169-169	1.000000	79.9
trnI-GAU-rns_p5	trnI-GAU-rns	5	CTGGGCCATTCTGGATTTGA	TGACTGGGGTGAAGTCGTAA	20	20	50.0	50.0	57.9	58.3	6.7	0.0	0.441339	128291	128346	193-193	1.000000	79.4