Hotspot

ycf1-chlN

Genome · 110034-110154 · 121 bp

Back to Porella

Pi 0.0639

MarkerSeek score 77.70

Primer available yes

Label ycf1-chlN

Feature Metadata

label_name: ycf1-chlN
feature_type: igs
region: Genome
length_bp: 121
variable_sites: 11
pi: 0.0639
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-chlN_p1	TCTTGCCAATACGGGACTTG	CAGATCTACCGGCCTTAGGA	50.0	55.0	57.9	58.0	1257-1266 bp	1.0	82.5
ycf1-chlN_p2	AGTGTGGTGAGGAATCCGTA	CAGATCTACCGGCCTTAGGA	50.0	55.0	58.1	58.0	914-923 bp	1.0	82.2
ycf1-chlN_p3	AGTGTGGTGAGGAATCCGTA	GGGTTGGTTACCTGCTCAAA	50.0	50.0	58.1	58.0	940-949 bp	1.0	82.1
ycf1-chlN_p4	TCTTGCCAATACGGGACTTG	TTCGGTGTGGAATGATCGTC	50.0	50.0	57.9	58.0	948-957 bp	0.666667	68.8
ycf1-chlN_p5	AGTGTGGTGAGGAATCCGTA	TTCGGTGTGGAATGATCGTC	50.0	50.0	58.1	58.0	605-614 bp	0.666667	68.6