Hotspot

rpl32-trnL-UAG

Genome · 95839-96624 · 786 bp

Back to Porella

Pi 0.0576

MarkerSeek score 81.70

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: Genome
length_bp: 786
variable_sites: 65
pi: 0.0576
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 81.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TGGGGCATCGACAAAAATCA	CTAACATTGCACCAGGAGCT	45.0	50.0	58.1	57.9	1712-1746 bp	0.666667	70.8
rpl32-trnL-UAG_p2	TGGGGCATCGACAAAAATCA	CGGAGCCAACTAGCTGAAAT	45.0	50.0	58.1	58.0	1388 bp	0.333333	52.4
rpl32-trnL-UAG_p3	AGACACGACGTAAATTGGGG	CGGAGCCAACTAGCTGAAAT	50.0	50.0	57.9	58.0	1403 bp	0.333333	52.4
rpl32-trnL-UAG_p4	TGGGGCATCGACAAAAATCA	GAGCTAACATTGCACCAGGA	45.0	50.0	58.1	57.9	1749 bp	0.333333	51.8
rpl32-trnL-UAG_p5	AGACACGACGTAAATTGGGG	GAGCTAACATTGCACCAGGA	50.0	50.0	57.9	57.9	1764 bp	0.333333	51.7