Hotspot

psaJ-rpl33

Genome · 66979-67129 · 151 bp

Back to Porella

Pi 0.0626

MarkerSeek score 80.50

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: Genome
length_bp: 151
variable_sites: 14
pi: 0.0626
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	ACGTTGTGGTTCGGATTCTT	CGACGACATCTTTTTCCAAA	45.0	40.0	57.8	54.6	318-319 bp	1.0	55.1
psaJ-rpl33_p2	CGGATTCTTGGCCGGTTTAT	CGACGACATCTTTTTCCAAA	50.0	40.0	58.3	54.6	307-308 bp	1.0	54.6
psaJ-rpl33_p3	ACGTTGTGGTTCGGATTCTT	CGACGACATCTTTTTCCAAAA	45.0	38.1	57.8	55.4	318-319 bp	1.0	53.6
psaJ-rpl33_p4	CGGATTCTTGGCCGGTTTAT	CGACGACATCTTTTTCCAAAA	50.0	38.1	58.3	55.4	307-308 bp	1.0	53.2
psaJ-rpl33_p5	GCTACGTTGTGGTTCGGATT	CGACGACATCTTTTTCCAAA	50.0	40.0	58.6	54.6	321-322 bp	1.0	52.5