Hotspot

psbJ-psbL

Genome · 64672-64791 · 120 bp

Back to Porella

Pi 0.0667

MarkerSeek score 77.70

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: Genome
length_bp: 120
variable_sites: 12
pi: 0.0667
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	CTTCCGGTAGTATTGGCCAT	TTTGTACTTGCTGTTCTTTTT	50.0	28.6	57.1	52.4	182-185 bp	1.0	46.1
psbJ-psbL_p2	CCTTCCGGTAGTATTGGCCA	TTTGTACTTGCTGTTCTTTTT	55.0	28.6	59.2	52.4	183-186 bp	1.0	46.1
psbJ-psbL_p3	CTTCCGGTAGTATTGGCCAT	ATTTGTACTTGCTGTTCTTTT	50.0	28.6	57.1	52.1	183-186 bp	1.0	46.1
psbJ-psbL_p4	TCCTTCCGGTAGTATTGGCC	TTTGTACTTGCTGTTCTTTTT	55.0	28.6	58.9	52.4	184-187 bp	1.0	46.0
psbJ-psbL_p5	TCCTTCCGGTAGTATTGGCC	ATTTGTACTTGCTGTTCTTTT	55.0	28.6	58.9	52.1	185-188 bp	1.0	46.0