Hotspot

trnT-UGU-trnL-UAA

Genome · 51644-51887 · 244 bp

Back to Porella

Pi 0.0558

MarkerSeek score 79.20

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: Genome
length_bp: 244
variable_sites: 19
pi: 0.0558
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GACCTCTGAGCTAAACAGGC	AAATTAAGTCCGCAGCGTCT	55.0	45.0	58.0	57.9	295-304 bp	1.0	87.7
trnT-UGU-trnL-UAA_p2	GACCTCTGAGCTAAACAGGC	ATTAAGTCCGCAGCGTCTAC	55.0	50.0	58.0	57.8	293-302 bp	1.0	86.9
trnT-UGU-trnL-UAA_p3	GACCTCTGAGCTAAACAGGC	TACCAATTTCGCCATACCCC	55.0	50.0	58.0	57.6	276-285 bp	1.0	86.2
trnT-UGU-trnL-UAA_p4	GAGTCGAACCGATGACCATC	AAATTAAGTCCGCAGCGTCT	55.0	45.0	58.2	57.9	336-345 bp	1.0	85.5
trnT-UGU-trnL-UAA_p5	GAGTCGAACCGATGACCATC	ATTAAGTCCGCAGCGTCTAC	55.0	50.0	58.2	57.8	334-343 bp	1.0	84.7