Hotspot

trnE-UGA-trnT-GGU

Genome · 37919-39170 · 1252 bp

Back to Porella

Pi 0.0451

MarkerSeek score 82.40

Primer available yes

Label trnE-UGA-trnT-GGU

Feature Metadata

label_name: trnE-UGA-trnT-GGU
feature_type: igs
region: Genome
length_bp: 1252
variable_sites: 76
pi: 0.0451
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UGA-trnT-GGU_p1	GTCGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	1346-1360 bp	1.0	88.4
trnE-UGA-trnT-GGU_p2	GTCGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.0	58.1	1325-1339 bp	1.0	88.0
trnE-UGA-trnT-GGU_p3	GTCGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.0	58.1	1322-1336 bp	1.0	87.9
trnE-UGA-trnT-GGU_p4	TCGCCTCCTTGAAAGAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	1345-1359 bp	1.0	87.8
trnE-UGA-trnT-GGU_p5	GTCGCCTCCTTGAAAGAGAG	AGGCCCCTTATCAGATTCGA	55.0	50.0	58.0	57.9	1364-1378 bp	1.0	87.6