Hotspot

chlB-trnK

Genome · 26430-27112 · 683 bp

Back to Porella

Pi 0.0444

MarkerSeek score 75.40

Primer available yes

Label chlB-trnK

Feature Metadata

label_name: chlB-trnK
feature_type: igs
region: Genome
length_bp: 683
variable_sites: 41
pi: 0.0444
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 75.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlB-trnK_p1	GCGGTAGTTTTTGGTGATGC	CAAGTCGCACTTAAAAGCCG	50.0	50.0	58.0	58.0	1294-1351 bp	1.0	86.9
chlB-trnK_p2	GCCCGACAAAATGGTATTGC	GTTGGTCAGCACACAGGTTA	50.0	50.0	58.1	58.0	1111-1168 bp	1.0	86.5
chlB-trnK_p3	CCTTCGGCAATTTTTGGGAC	GTTGGTCAGCACACAGGTTA	50.0	50.0	57.9	58.0	1459-1516 bp	1.0	85.7
chlB-trnK_p4	CTTCGGCAATTTTTGGGACC	GTTGGTCAGCACACAGGTTA	50.0	50.0	57.9	58.0	1458-1515 bp	1.0	85.7
chlB-trnK_p5	GCGGTAGTTTTTGGTGATGC	GTTGGTCAGCACACAGGTTA	50.0	50.0	58.0	58.0	1660-1717 bp	1.0	85.6