Hotspot

psaM-trnS-GCU

Genome · 23279-23666 · 388 bp

Back to Porella

Pi 0.0685

MarkerSeek score 81.50

Primer available yes

Label psaM-trnS-GCU

Feature Metadata

label_name: psaM-trnS-GCU
feature_type: igs
region: Genome
length_bp: 388
variable_sites: 36
pi: 0.0685
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaM-trnS-GCU_p1	CGTTACAGGTATTTTGGCCC	GCTCGTACAACGGATTAGCA	50.0	50.0	56.8	58.1	466-490 bp	1.0	79.7
psaM-trnS-GCU_p2	CGTTACAGGTATTTTGGCCCT	GCTCGTACAACGGATTAGCA	47.6	50.0	58.3	58.1	466-490 bp	1.0	79.1
psaM-trnS-GCU_p3	TCGTTACAGGTATTTTGGCCC	GCTCGTACAACGGATTAGCA	47.6	50.0	58.3	58.1	467-491 bp	1.0	79.1
psaM-trnS-GCU_p4	CGTTACAGGTATTTTGGCCC	AGCTCGTACAACGGATTAGC	50.0	50.0	56.8	57.8	467-491 bp	1.0	78.7
psaM-trnS-GCU_p5	CGTTACAGGTATTTTGGCCCT	AGCTCGTACAACGGATTAGC	47.6	50.0	58.3	57.8	467-491 bp	1.0	78.2