Hotspot

trnR-UCU-trnG-GCC

Genome · 21916-21994 · 79 bp

Back to Porella

Pi 0.0591

MarkerSeek score 75.90

Primer available yes

Label trnR-UCU-trnG-GCC

Feature Metadata

label_name: trnR-UCU-trnG-GCC
feature_type: igs
region: Genome
length_bp: 79
variable_sites: 7
pi: 0.0591
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-trnG-GCC_p1	GGATTCGAACCTATACTGGAGG	TTCCAAGCTAATGATGCGGG	50.0	50.0	57.5	58.3	179 bp	1.0	60.4
trnR-UCU-trnG-GCC_p2	GGATTCGAACCTATACTGGAGG	GCTAATGATGCGGGTTCGAT	50.0	50.0	57.5	58.5	173 bp	1.0	59.2
trnR-UCU-trnG-GCC_p3	GGATTCGAACCTATACTGGAGG	CCAAGCTAATGATGCGGGTT	50.0	50.0	57.5	58.6	177 bp	1.0	58.1
trnR-UCU-trnG-GCC_p4	GGATTCGAACCTATACTGGAGG	AATGATGCGGGTTCGATTCC	50.0	50.0	57.5	58.7	170 bp	1.0	57.6
trnR-UCU-trnG-GCC_p5	GGATTCGAACCTATACTGGAGG	AAGCTAATGATGCGGGTTCG	50.0	50.0	57.5	58.7	175 bp	1.0	57.4