Hotspot

ycf66-petN

Genome · 5347-5430 · 84 bp

Back to Porella

Pi 0.0794

MarkerSeek score 76.10

Primer available yes

Label ycf66-petN

Feature Metadata

label_name: ycf66-petN
feature_type: igs
region: Genome
length_bp: 84
variable_sites: 10
pi: 0.0794
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf66-petN_p1	ATCGACTTGGATTTCGGGTG	ATAAAACAAACGGCGGCATG	50.0	45.0	58.0	58.0	997-1005 bp	1.0	83.6
ycf66-petN_p2	ATCGACTTGGATTTCGGGTG	AGGATCAAACCGTCATGCTC	50.0	50.0	58.0	58.0	871-876 bp	1.0	83.6
ycf66-petN_p3	ATCGACTTGGATTTCGGGTG	GGATCAAACCGTCATGCTCT	50.0	50.0	58.0	58.0	870-875 bp	1.0	83.6
ycf66-petN_p4	ATCGACTTGGATTTCGGGTG	TCGTATGGGGAAGAAGTGGT	50.0	50.0	58.0	58.1	536-538 bp	1.0	83.3
ycf66-petN_p5	ATCGACTTGGATTTCGGGTG	GGGTGTCGCTTCATTTTTCG	50.0	50.0	58.0	58.0	931-934 bp	0.666667	68.8