Hotspot

psaC

SSC · 117843-118088 · 246 bp

Back to Populus

Pi 0.00474

MarkerSeek score 29.50

Primer available yes

Label psaC

Feature Metadata

label_name: psaC
feature_type: gene
region: SSC
length_bp: 246
variable_sites: 7
pi: 0.00474
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 29.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC_p1	GAGTACGGGTTTTTGTCGGT	CTAGATCGAAAATTTATGACGT	50.0	31.8	58.1	52.2	352-359 bp	1.0	43.2
psaC_p2	GAGTACGGGTTTTTGTCGGT	ACTAGATCGAAAATTTATGACGT	50.0	30.4	58.1	54.0	353-360 bp	1.0	43.1
psaC_p3	GAGTACGGGTTTTTGTCGGT	ACTAGATCGAAAATTTATGACG	50.0	31.8	58.1	52.2	353-360 bp	1.0	43.1
psaC_p4	GAGTACGGGTTTTTGTCGGTA	ACTAGATCGAAAATTTATGACGT	47.6	30.4	58.0	54.0	353-360 bp	1.0	43.1
psaC_p5	GAGTACGGGTTTTTGTCGGT	ACTAGATCGAAAATTTATGACGTA	50.0	29.2	58.1	54.0	353-360 bp	1.0	43.1