Hotspot

ndhF

SSC · 111944-114232 · 2289 bp

Back to Populus

Pi 0.00435

MarkerSeek score 43.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2289
variable_sites: 110
pi: 0.00435
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GTGGGCTCTATCGTGGAAAA	TTTCGAGTTCTGTTCCGGAC	50.0	50.0	57.6	57.9	2770-2947 bp	0.877193	79.3
ndhF_p2	GTGGGCTCTATCGTGGAAAA	CGAGTTCTGTTCCGGACTTT	50.0	50.0	57.6	57.8	2767-2944 bp	0.877193	79.3
ndhF_p3	AGTGGGCTCTATCGTGGAAA	TTTCGAGTTCTGTTCCGGAC	50.0	50.0	58.4	57.9	2771-2948 bp	0.877193	79.0
ndhF_p4	GTGGGCTCTATCGTGGAAAA	GTTTCGAGTTCTGTTCCGGA	50.0	50.0	57.6	57.9	2771-2948 bp	0.859649	78.6
ndhF_p5	GTGGGCTCTATCGTGGAAAA	AGTTTCGAGTTCTGTTCCGG	50.0	50.0	57.6	57.8	2772-2927 bp	0.491228	61.3