Hotspot

rpl36-rps8

LSC · 79839-80315 · 477 bp

Back to Populus

Pi 0.0074

MarkerSeek score 62.70

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 477
variable_sites: 37
pi: 0.0074
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	TTTATGTCTCGGGTTGGAGC	CGGGGTATAATGACAGACCG	50.0	55.0	57.6	57.6	650-680 bp	1.0	81.6
rpl36-rps8_p2	GTCTCGGGTTGGAGCAAATT	CGGGGTATAATGACAGACCG	50.0	55.0	58.5	57.6	645-675 bp	1.0	81.1
rpl36-rps8_p3	ATTAGAATTCGTCCCCGTCG	CGGGGTATAATGACAGACCG	50.0	55.0	57.5	57.6	625-655 bp	1.0	81.1
rpl36-rps8_p4	TTAGAATTCGTCCCCGTCGA	CGGGGTATAATGACAGACCG	50.0	55.0	58.5	57.6	624-654 bp	1.0	80.8
rpl36-rps8_p5	TCCCCGTCGACGTATTAGTC	CGGGGTATAATGACAGACCG	55.0	55.0	58.7	57.6	614-644 bp	1.0	79.7