Hotspot

psbH-petD

LSC · 74857-77423 · 2567 bp

Back to Populus

Pi 0.00263
MarkerSeek score 59.80
Primer available yes
Label psbH-petD

Feature Metadata

label_name
psbH-petD
feature_type
igs
region
LSC
length_bp
2567
variable_sites
71
pi
0.00263
unique_haplotype_count
41
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbH-petD_p1 GTCGCAATGGCTCTATTTGC TGACCCATTCCTTTAGCCAA 50.0 45.0 57.9 56.7 2688-2800 bp 1.0 75.2
psbH-petD_p2 CTTTGATGGGTGTCGCAATG TGACCCATTCCTTTAGCCAA 50.0 45.0 57.7 56.7 2699-2811 bp 1.0 73.9
psbH-petD_p3 GGTGTCGCAATGGCTCTATT TGACCCATTCCTTTAGCCAA 50.0 45.0 58.3 56.7 2691-2803 bp 1.0 73.4
psbH-petD_p4 GTCGCAATGGCTCTATTTGC ATGACCCATTCCTTTAGCCA 50.0 45.0 57.9 56.5 2689-2801 bp 1.0 73.3
psbH-petD_p5 GGTGGGGAACTACTCCTTTG TGACCCATTCCTTTAGCCAA 55.0 45.0 57.5 56.7 2714-2826 bp 1.0 72.1