Hotspot

psbJ-psbL

LSC · 63727-63913 · 187 bp

Back to Populus

Pi 0.0274

MarkerSeek score 69.90

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: LSC
length_bp: 187
variable_sites: 28
pi: 0.0274
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	TCCTTCCAGTAGTATCGGCC	ACACAATCAAACCCGAACGA	55.0	45.0	58.3	58.0	303-411 bp	1.0	88.2
psbJ-psbL_p2	AAGCCGGAATAGGAGCCATA	ACACAATCAAACCCGAACGA	50.0	45.0	58.3	58.0	388-496 bp	1.0	87.2
psbJ-psbL_p3	AGCCGGAATAGGAGCCATAA	ACACAATCAAACCCGAACGA	50.0	45.0	58.3	58.0	387-495 bp	1.0	87.2
psbJ-psbL_p4	GATGAACCCAAGCCGGAATA	ACACAATCAAACCCGAACGA	50.0	45.0	57.7	58.0	397-505 bp	1.0	86.5
psbJ-psbL_p5	ATGAACCCAAGCCGGAATAG	ACACAATCAAACCCGAACGA	50.0	45.0	57.7	58.0	396-504 bp	1.0	86.5