Hotspot

ycf4-cemA

LSC · 60392-60766 · 375 bp

Back to Populus

Pi 0.0114

MarkerSeek score 58.90

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 375
variable_sites: 28
pi: 0.0114
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GGCCATTCCTTTGACTCGTA	TGAAAAAGAGACCCACCAGG	50.0	50.0	57.6	57.3	541-585 bp	1.0	80.1
ycf4-cemA_p2	ACTCCACGAGAAATTGAGCA	TGAAAAAGAGACCCACCAGG	45.0	50.0	57.2	57.3	507-551 bp	1.0	76.7
ycf4-cemA_p3	GGCCATTCCTTTGACTCGTA	ATGAAAAAGAGACCCACCAGG	50.0	47.6	57.6	57.8	542-586 bp	1.0	76.1
ycf4-cemA_p4	GCCATTCCTTTGACTCGTACT	TGAAAAAGAGACCCACCAGG	47.6	50.0	58.0	57.3	540-584 bp	1.0	75.1
ycf4-cemA_p5	GGGGCCATTCCTTTGACTC	TGAAAAAGAGACCCACCAGG	57.9	50.0	58.1	57.3	543-587 bp	1.0	74.4