Hotspot

trnF-GAA-ndhJ

LSC · 47425-48170 · 746 bp

Back to Populus

Pi 0.00866

MarkerSeek score 62.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 746
variable_sites: 42
pi: 0.00866
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCAGGTAGA	CCGGAAAGTTGGATAGGGTG	55.0	55.0	58.0	58.0	490-920 bp	1.0	87.6
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCAGGTAGA	CGGAAAGTTGGATAGGGTGG	55.0	55.0	58.0	58.0	489-919 bp	1.0	87.6
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	454-884 bp	1.0	87.2
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CGGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	453-883 bp	1.0	87.2
trnF-GAA-ndhJ_p5	CAGGTAGAGCAGAGGACTGA	CGGAAAGTTGGATAGGGTGG	55.0	55.0	57.9	58.0	477-907 bp	1.0	86.5