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psbM-trnD-GUC

LSC · 28698-29897 · 1200 bp

Back to Populus

Pi 0.00509

MarkerSeek score 58.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1200
variable_sites: 63
pi: 0.00509
unique_haplotype_count: 39
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.8	58.5	1232-1436 bp	1.0	66.1
psbM-trnD-GUC_p2	AGAATGAACAGTGCAGTAGC	TTTCGTTTCCCTGGGATTGT	45.0	45.0	55.8	57.3	1254-1458 bp	0.982456	63.7
psbM-trnD-GUC_p3	AGGAACTAGAATGAACAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	57.4	58.5	1239-1443 bp	1.0	62.9
psbM-trnD-GUC_p4	AGAATGAACAGTGCAGTAGC	CGTTTCCCTGGGATTGTAGT	45.0	50.0	55.8	57.2	1251-1455 bp	0.982456	62.8
psbM-trnD-GUC_p5	AGAATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	45.0	50.0	55.8	57.0	1233-1437 bp	1.0	62.0