Hotspot

atpA-atpF

LSC · 10341-10400 · 60 bp

Back to Populus

Pi 0.0747

MarkerSeek score 72.10

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 13
pi: 0.0747
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	TACTAATCTCGTCGGCTCGA	TGTTTGGGGCGATGAAAGAA	50.0	45.0	57.8	58.0	113-125 bp	1.0	90.1
atpA-atpF_p2	CACTTGAAGTACGGTCCCAG	TGTTTGGGGCGATGAAAGAA	55.0	45.0	57.9	58.0	187-199 bp	1.0	89.8
atpA-atpF_p3	CACCCACTTGAAGTACGGTC	TGTTTGGGGCGATGAAAGAA	55.0	45.0	58.2	58.0	191-203 bp	1.0	88.7
atpA-atpF_p4	CCGTCACCCACTTGAAGTAC	TGTTTGGGGCGATGAAAGAA	55.0	45.0	58.2	58.0	195-207 bp	1.0	88.6
atpA-atpF_p5	GTCACCCACTTGAAGTACGG	TGTTTGGGGCGATGAAAGAA	55.0	45.0	58.2	58.0	193-205 bp	1.0	88.6