Hotspot

trnS-GCU-trnR-UCU

LSC · 6959-8641 · 1683 bp

Back to Populus

Pi 0.0138

MarkerSeek score 60.60

Primer available yes

Label trnS-GCU-trnR-UCU

Feature Metadata

label_name: trnS-GCU-trnR-UCU
feature_type: igs
region: LSC
length_bp: 1683
variable_sites: 141
pi: 0.0138
unique_haplotype_count: 44
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnR-UCU_p1	GCTTTAGTCCACTCAGCCAT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.6	56.9	1710-1902 bp	1.0	70.6
trnS-GCU-trnR-UCU_p2	GCTTTAGTCCACTCAGCCAT	AGGTTTAGAAGACCTCTGTCCT	50.0	45.5	57.6	57.9	1716-1908 bp	1.0	69.9
trnS-GCU-trnR-UCU_p3	AGTCCACTCAGCCATCTCTC	AGAAGACCTCTGTCCTATCCA	55.0	47.6	58.5	56.9	1705-1897 bp	1.0	69.8
trnS-GCU-trnR-UCU_p4	AGTCCACTCAGCCATCTCTC	AGGTTTAGAAGACCTCTGTCCT	55.0	45.5	58.5	57.9	1711-1903 bp	1.0	69.1
trnS-GCU-trnR-UCU_p5	TAGTCCACTCAGCCATCTCT	AGAAGACCTCTGTCCTATCCA	50.0	47.6	57.2	56.9	1706-1898 bp	1.0	67.2