Hotspot

psaC-ndhE

SSC · 123231-123887 · 657 bp

Back to Pontederia

Pi 0.0558

MarkerSeek score 84.90

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 657
variable_sites: 174
pi: 0.0558
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 84.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CATAAATTTTTACTGAATGTGACA	CGATCCACCGGAACAGAAAA	25.0	50.0	52.3	58.2	678-822 bp	1.0	52.0
psaC-ndhE_p2	TCATAAATTTTTACTGAATGTGAC	CGATCCACCGGAACAGAAAA	25.0	50.0	52.1	58.2	679-823 bp	1.0	52.0
psaC-ndhE_p3	CATAAATTTTTACTGAATGTGACA	CTGAAGCAGCTATTGGGCTA	25.0	50.0	52.3	57.4	711-855 bp	1.0	52.0
psaC-ndhE_p4	CATAAATTTTTACTGAATGTGACA	TGAAGCAGCTATTGGGCTAG	25.0	50.0	52.3	57.4	710-854 bp	1.0	52.0
psaC-ndhE_p5	CATAAATTTTTACTGAATGTGACA	ATTGTTTCGTCGATCCACCG	25.0	50.0	52.3	58.6	688-832 bp	1.0	52.0