Hotspot

ndhF-rpl32

SSC · 118312-119199 · 888 bp

Back to Pontederia

Pi 0.0316

MarkerSeek score 73.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 888
variable_sites: 155
pi: 0.0316
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TTTTGACAAGCACTTGCAGC	AAAGGGCTTTTACTGCAGCT	45.0	45.0	58.1	58.0	1515-1629 bp	0.961538	89.9
ndhF-rpl32_p2	CTCCCACGAGTTCCCAAAAA	AAAGGGCTTTTACTGCAGCT	50.0	45.0	58.0	58.0	1451-1565 bp	0.923077	88.7
ndhF-rpl32_p3	ATGCTTTTTGTTGCTGTCGG	AAAGGGCTTTTACTGCAGCT	45.0	45.0	57.9	58.0	1092-1206 bp	0.884615	86.3
ndhF-rpl32_p4	ACAAGCACTTGCAGCAATTG	AAAGGGCTTTTACTGCAGCT	45.0	45.0	58.1	58.0	1510-1563 bp	0.269231	54.3
ndhF-rpl32_p5	GACAAGCACTTGCAGCAATT	AAAGGGCTTTTACTGCAGCT	45.0	45.0	57.9	58.0	1511-1564 bp	0.269231	54.3