Hotspot

petD-rpoA

LSC · 83094-83267 · 174 bp

Back to Pontederia

Pi 0.0515

MarkerSeek score 74.20

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 174
variable_sites: 43
pi: 0.0515
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TAATGGTTTCAGTACCGGCG	AGAGATGCATCCATTTGCGT	50.0	45.0	58.0	58.0	1065-1100 bp	1.0	89.4
petD-rpoA_p2	TAATGGTTTCAGTACCGGCG	CATTTGCGTCAAGGGTCCTA	50.0	50.0	58.0	57.9	1054-1089 bp	1.0	89.0
petD-rpoA_p3	CTCGAGCCGGATGATGAAAA	AGAGATGCATCCATTTGCGT	50.0	45.0	58.1	58.0	1435-1470 bp	1.0	87.6
petD-rpoA_p4	TAATGGTTTCAGTACCGGCG	GGATACCGTACGAAACCCAC	50.0	55.0	58.0	58.1	909-944 bp	0.923077	85.2
petD-rpoA_p5	CTCGAGCCGGATGATGAAAA	GGATACCGTACGAAACCCAC	50.0	55.0	58.1	58.1	1279-1314 bp	0.923077	83.5