Hotspot

psaJ-rpl33

LSC · 72360-72980 · 621 bp

Back to Pontederia

Pi 0.0505

MarkerSeek score 87.40

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 621
variable_sites: 123
pi: 0.0505
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 87.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TGGTTTGGGTCTTTAGCAGG	AATGCCCGGCGATTCTTTAT	50.0	45.0	57.7	58.0	722-888 bp	1.0	89.6
psaJ-rpl33_p2	TGGTTTGGGTCTTTAGCAGG	ATGCCCGGCGATTCTTTATT	50.0	45.0	57.7	58.0	721-887 bp	1.0	89.6
psaJ-rpl33_p3	GGTTTGGGTCTTTAGCAGGT	AATGCCCGGCGATTCTTTAT	50.0	45.0	57.7	58.0	721-887 bp	1.0	89.5
psaJ-rpl33_p4	GGTTTGGGTCTTTAGCAGGT	ATGCCCGGCGATTCTTTATT	50.0	45.0	57.7	58.0	720-886 bp	1.0	89.5
psaJ-rpl33_p5	TGGTTTGGGTCTTTAGCAGG	AAATGCCCGGCGATTCTTTA	50.0	45.0	57.7	58.2	723-889 bp	1.0	87.8