Hotspot

trnF-GAA-ndhJ

LSC · 51524-52213 · 690 bp

Back to Pontederia

Pi 0.027

MarkerSeek score 75.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 690
variable_sites: 101
pi: 0.027
unique_haplotype_count: 24
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	ATAGGTTGGCCCCTACGTAA	50.0	50.0	58.1	57.8	776-811 bp	0.961538	88.6
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AAAGTTGGATAGGTTGGCCC	50.0	50.0	58.1	57.8	784-819 bp	0.961538	88.2
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	GATAGGTTGGCCCCTACGTA	50.0	55.0	58.1	58.3	777-812 bp	0.923077	86.2
trnF-GAA-ndhJ_p4	CGGGAAATAGTCGGGATAGC	ATAGGTTGGCCCCTACGTAA	55.0	50.0	57.6	57.8	824-859 bp	0.961538	86.1
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	TAGGTTGGCCCCTACGTAAG	50.0	55.0	58.1	58.5	775-810 bp	0.923077	84.4