Hotspot

rpoB-trnC-GCA

LSC · 29211-30385 · 1175 bp

Back to Pontederia

Pi 0.0241

MarkerSeek score 74.20

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1175
variable_sites: 151
pi: 0.0241
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTTCCATCATTCCGGAGCA	GCGACACCCAGATTTGAACT	45.0	50.0	57.3	58.5	1265-1360 bp	0.923077	79.2
rpoB-trnC-GCA_p2	TTTCCATCATTCCGGAGCAT	GCGACACCCAGATTTGAACT	45.0	50.0	57.3	58.5	1264-1359 bp	0.923077	79.2
rpoB-trnC-GCA_p3	ATTTCCATCATTCCGGAGCA	TAAAGGATTTGCAGTCCCCC	45.0	50.0	57.3	57.5	1240-1335 bp	0.923077	78.8
rpoB-trnC-GCA_p4	TTTCCATCATTCCGGAGCAT	TAAAGGATTTGCAGTCCCCC	45.0	50.0	57.3	57.5	1239-1334 bp	0.923077	78.8
rpoB-trnC-GCA_p5	TTTCCATCATTCCGGAGCAT	CCTTACCACTTGGCCATGTC	45.0	55.0	57.3	58.5	1218-1313 bp	0.923077	78.8