Hotspot

trnS-GCU-trnG-UCC

LSC · 8848-10345 · 1498 bp

Back to Pontederia

Pi 0.114

MarkerSeek score 76.90

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 1498
variable_sites: 580
pi: 0.114
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CCACTCAGCCATCTCTCC	ACGAATCACACTTTTACCACT	61.1	38.1	56.4	55.5	1024-2151 bp	0.961538	50.5
trnS-GCU-trnG-UCC_p2	CCACTCAGCCATCTCTCC	AGAACGAATCACACTTTTACCAC	61.1	39.1	56.4	57.3	1027-2154 bp	0.961538	50.5
trnS-GCU-trnG-UCC_p3	CCACTCAGCCATCTCTCC	GAACGAATCACACTTTTACCACT	61.1	39.1	56.4	57.3	1026-2153 bp	0.961538	50.5
trnS-GCU-trnG-UCC_p4	CCACTCAGCCATCTCTCC	AACGAATCACACTTTTACCACT	61.1	36.4	56.4	56.2	1025-2152 bp	0.961538	50.5
trnS-GCU-trnG-UCC_p5	CCACTCAGCCATCTCTCC	TACCACTAAACTATACCCGC	61.1	45.0	56.4	54.0	1010-2137 bp	0.961538	50.5