Hotspot

ycf1

SSC · 116066-116134 · 69 bp

Back to Pomatocalpa

Pi 0.585

MarkerSeek score 77.10

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 69
variable_sites: 38
pi: 0.585
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TTGCACGACACTAGCAACAT	ATTCGTAGACCCCTTTGTGA	45.0	45.0	58.1	56.2	986 bp	0.5	44.4
ycf1_p2	TTGCACGACACTAGCAACAT	TTCGTAGACCCCTTTGTGAT	45.0	45.0	58.1	56.2	985 bp	0.5	44.4
ycf1_p3	TTGCACGACACTAGCAACAT	TCCACTCTTCCTAATGCACA	45.0	45.0	58.1	56.2	542 bp	0.5	44.3
ycf1_p4	AGGGACCTAGTTCTTGCAGA	ATTCGTAGACCCCTTTGTGA	50.0	45.0	57.7	56.2	1180 bp	0.5	42.7
ycf1_p5	AGGGACCTAGTTCTTGCAGA	TTCGTAGACCCCTTTGTGAT	50.0	45.0	57.7	56.2	1179 bp	0.5	42.7