Hotspot

ndhG

SSC · 117054-117320 · 267 bp

Back to Pomatocalpa

Pi 0.498

MarkerSeek score 77.00

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 267
variable_sites: 127
pi: 0.498
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 77.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	GCCACAAGGTACATTGGACA	CCTATCTCCAAAGTTCCGCA	50.0	50.0	58.1	57.3	350 bp	0.5	53.7
ndhG_p2	TGAGCCACAAGGTACATTGG	CCTATCTCCAAAGTTCCGCA	50.0	50.0	57.8	57.3	353 bp	0.5	52.9
ndhG_p3	AGCCACAAGGTACATTGGAC	CCTATCTCCAAAGTTCCGCA	50.0	50.0	57.8	57.3	351 bp	0.5	52.9
ndhG_p4	GCCACAAGGTACATTGGACA	TCCTATCTCCAAAGTTCCGC	50.0	50.0	58.1	57.0	351 bp	0.5	51.4
ndhG_p5	GCCACAAGGTACATTGGACA	CCTATCTCCAAAGTTCCGCAA	50.0	47.6	58.1	58.0	350 bp	0.5	51.1