Hotspot

trnL-UAG-trnN-GUU

SSC · 121165-122732 · 1568 bp

Back to Pomatocalpa

Pi 0.323

MarkerSeek score 82.80

Primer available yes

Label trnL-UAG-trnN-GUU

Feature Metadata

label_name: trnL-UAG-trnN-GUU
feature_type: igs
region: SSC
length_bp: 1568
variable_sites: 469
pi: 0.323
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-trnN-GUU_p1	TGGCGAAACCTTGGGTAAAA	GGCAGAATAGCAGAGCAAGT	45.0	50.0	57.9	58.0	2565 bp	0.5	59.2
trnL-UAG-trnN-GUU_p2	GCGGGTTGATTTGCATTTCA	GGCAGAATAGCAGAGCAAGT	45.0	50.0	57.9	58.0	2601 bp	0.5	59.1
trnL-UAG-trnN-GUU_p3	TGCGGGTTGATTTGCATTTC	GGCAGAATAGCAGAGCAAGT	45.0	50.0	57.9	58.0	2602 bp	0.5	59.1
trnL-UAG-trnN-GUU_p4	AAGTTCCTAAGAGCAGCGTG	GGCAGAATAGCAGAGCAAGT	50.0	50.0	57.9	58.0	2005 bp	0.5	59.0
trnL-UAG-trnN-GUU_p5	TGGCGAAACCTTGGGTAAAA	AAGCGAGCCCTTTCATTCAA	45.0	45.0	57.9	58.1	2288 bp	0.5	58.7