Hotspot

ccsA-trnL-UAG

SSC · 120920-121069 · 150 bp

Back to Pomatocalpa

Pi 0.404

MarkerSeek score 75.10

Primer available yes

Label ccsA-trnL-UAG

Feature Metadata

label_name: ccsA-trnL-UAG
feature_type: igs
region: SSC
length_bp: 150
variable_sites: 59
pi: 0.404
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 75.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-trnL-UAG_p1	TGGCGAAACCTTGGGTAAAA	CACGCTGCTCTTAGGAACTT	45.0	50.0	57.9	57.9	580 bp	0.5	58.8
ccsA-trnL-UAG_p2	GCGGGTTGATTTGCATTTCA	CACGCTGCTCTTAGGAACTT	45.0	50.0	57.9	57.9	616 bp	0.5	58.7
ccsA-trnL-UAG_p3	TGCGGGTTGATTTGCATTTC	CACGCTGCTCTTAGGAACTT	45.0	50.0	57.9	57.9	617 bp	0.5	58.7
ccsA-trnL-UAG_p4	TGGCGAAACCTTGGGTAAAA	AGACTCCGACTGAGAACGAA	45.0	50.0	57.9	58.1	881 bp	0.5	58.6
ccsA-trnL-UAG_p5	GCGGGTTGATTTGCATTTCA	AGACTCCGACTGAGAACGAA	45.0	50.0	57.9	58.1	917 bp	0.5	58.5