Hotspot

ndhG-psaC

SSC · 117321-118257 · 937 bp

Back to Pomatocalpa

Pi 0.47

MarkerSeek score 80.60

Primer available yes

Label ndhG-psaC

Feature Metadata

label_name: ndhG-psaC
feature_type: igs
region: SSC
length_bp: 937
variable_sites: 371
pi: 0.47
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-psaC_p1	GCCACAAGGTACATTGGACA	AGAGGTTTTCGGAGTCTCCA	50.0	50.0	58.1	58.0	1929 bp	0.5	59.1
ndhG-psaC_p2	GCCACAAGGTACATTGGACA	TCCAGAGGTTTTCGGAGTCT	50.0	50.0	58.1	58.0	1932 bp	0.5	59.1
ndhG-psaC_p3	TGCCACGCAGTTCCTTATTT	AGAGGTTTTCGGAGTCTCCA	45.0	50.0	58.1	58.0	1695 bp	0.5	59.1
ndhG-psaC_p4	TGCCACGCAGTTCCTTATTT	TCCAGAGGTTTTCGGAGTCT	45.0	50.0	58.1	58.0	1698 bp	0.5	59.1
ndhG-psaC_p5	TGCCACGCAGTTCCTTATTT	TTGGACAAGCGGATTCACAA	45.0	45.0	58.1	58.0	1214 bp	0.5	59.0