Hotspot

trnN-GUU-ycf1

SSC · 110149-116065 · 5917 bp

Back to Pomatocalpa

Pi 0.428

MarkerSeek score 76.80

Primer available yes

Label trnN-GUU-ycf1

Feature Metadata

label_name: trnN-GUU-ycf1
feature_type: igs
region: SSC
length_bp: 5917
variable_sites: 2353
pi: 0.428
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 76.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ycf1_p1	GCCGTCAGACAAGGAAGAAT	AGATTGCGACGAAAATCCGA	50.0	45.0	57.9	58.0	1369 bp	0.5	59.2
trnN-GUU-ycf1_p2	ACGGAAGAAGAGGGCAAAAG	AGATTGCGACGAAAATCCGA	50.0	45.0	58.1	58.0	1303 bp	0.5	59.1
trnN-GUU-ycf1_p3	GAAACGGAAGAAGAGGGCAA	AGATTGCGACGAAAATCCGA	50.0	45.0	58.1	58.0	1306 bp	0.5	59.1
trnN-GUU-ycf1_p4	GCCGTCAGACAAGGAAGAAT	TCCTCGATATGGTCCGTTGA	50.0	50.0	57.9	57.9	835 bp	0.5	58.7
trnN-GUU-ycf1_p5	ACGGAAGAAGAGGGCAAAAG	TCCTCGATATGGTCCGTTGA	50.0	50.0	58.1	57.9	769 bp	0.5	58.7