primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	psbK-psbI	1	GTTTGGCAAGCTGCTGTAAG	GCCCCGGATCGTTAGATAAG	20	20	50.0	55.0	57.9	57.6	1.4	0.7	0.421502	7726	8210	594-603	1.000000	79.4
psbK-psbI_p2	psbK-psbI	2	GTTTGGCAAGCTGCTGTAAG	TTCTTCACGCCCAGGATTAC	20	20	50.0	50.0	57.9	57.6	1.4	0.0	0.452684	7726	8210	614-623	1.000000	79.1
psbK-psbI_p3	psbK-psbI	3	TGGCAAGCTGCTGTAAGTTT	GCCCCGGATCGTTAGATAAG	20	20	45.0	55.0	58.3	57.6	1.4	0.7	0.674211	7726	8210	591-600	1.000000	77.4
psbK-psbI_p4	psbK-psbI	4	TTGGCAAGCTGCTGTAAGTT	GCCCCGGATCGTTAGATAAG	20	20	45.0	55.0	58.3	57.6	1.4	0.7	0.674211	7726	8210	592-601	1.000000	77.4
psbK-psbI_p5	psbK-psbI	5	TTTGGCAAGCTGCTGTAAGT	GCCCCGGATCGTTAGATAAG	20	20	45.0	55.0	58.3	57.6	1.4	0.7	0.674211	7726	8210	593-602	1.000000	77.4
trnN-GUU-ycf1_p1	trnN-GUU-ycf1	1	GCCGTCAGACAAGGAAGAAT	AGATTGCGACGAAAATCCGA	20	20	50.0	45.0	57.9	58.0	0.4	15.9	0.105187	111963	112082	1369-1369	0.500000	59.2
trnN-GUU-ycf1_p2	trnN-GUU-ycf1	2	ACGGAAGAAGAGGGCAAAAG	AGATTGCGACGAAAATCCGA	20	20	50.0	45.0	58.1	58.0	0.0	15.9	0.107023	111963	112082	1303-1303	0.500000	59.1
trnN-GUU-ycf1_p3	trnN-GUU-ycf1	3	GAAACGGAAGAAGAGGGCAA	AGATTGCGACGAAAATCCGA	20	20	50.0	45.0	58.1	58.0	0.0	15.9	0.112373	111963	112082	1306-1306	0.500000	59.1
trnN-GUU-ycf1_p4	trnN-GUU-ycf1	4	GCCGTCAGACAAGGAAGAAT	TCCTCGATATGGTCCGTTGA	20	20	50.0	50.0	57.9	57.9	0.4	0.0	0.162114	111963	112082	835-835	0.500000	58.7
trnN-GUU-ycf1_p5	trnN-GUU-ycf1	5	ACGGAAGAAGAGGGCAAAAG	TCCTCGATATGGTCCGTTGA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.163950	111963	112082	769-769	0.500000	58.7
ycf1_p1	ycf1	1	TTGCACGACACTAGCAACAT	ATTCGTAGACCCCTTTGTGA	20	20	45.0	45.0	58.1	56.2	0.0	0.0	1.955027	116066	116134	986-986	0.500000	44.4
ycf1_p2	ycf1	2	TTGCACGACACTAGCAACAT	TTCGTAGACCCCTTTGTGAT	20	20	45.0	45.0	58.1	56.2	0.0	0.0	1.955027	116066	116134	985-985	0.500000	44.4
ycf1_p3	ycf1	3	TTGCACGACACTAGCAACAT	TCCACTCTTCCTAATGCACA	20	20	45.0	45.0	58.1	56.2	0.0	0.0	1.962952	116066	116134	542-542	0.500000	44.3
ycf1_p4	ycf1	4	AGGGACCTAGTTCTTGCAGA	ATTCGTAGACCCCTTTGTGA	20	20	50.0	45.0	57.7	56.2	0.0	0.0	2.159990	116066	116134	1180-1180	0.500000	42.7
ycf1_p5	ycf1	5	AGGGACCTAGTTCTTGCAGA	TTCGTAGACCCCTTTGTGAT	20	20	50.0	45.0	57.7	56.2	0.0	0.0	2.159990	116066	116134	1179-1179	0.500000	42.7
ycf1-rps15_p1	ycf1-rps15	1	TTGCACGACACTAGCAACAT	AAATAAGGAACTGCGTGGCA	20	20	45.0	45.0	58.1	58.1	0.0	0.0	0.218076	116135	116584	1484-1484	0.500000	58.3
ycf1-rps15_p2	ycf1-rps15	2	TTGCACGACACTAGCAACAT	TGTCCAATGTACCTTGTGGC	20	20	45.0	50.0	58.1	58.1	0.0	9.6	0.218076	116135	116584	1250-1250	0.500000	58.3
ycf1-rps15_p3	ycf1-rps15	3	TTGCACGACACTAGCAACAT	CCAATGTACCTTGTGGCTCA	20	20	45.0	50.0	58.1	57.8	0.0	9.6	0.321190	116135	116584	1247-1247	0.500000	57.4
ycf1-rps15_p4	ycf1-rps15	4	TTGCACGACACTAGCAACAT	GTCCAATGTACCTTGTGGCT	20	20	45.0	50.0	58.1	57.8	0.0	9.6	0.322736	116135	116584	1249-1249	0.500000	57.4
ycf1-rps15_p5	ycf1-rps15	5	TTGCACGACACTAGCAACAT	CCGTTCATAAACAGCACAGC	20	20	45.0	50.0	58.1	57.8	0.0	0.0	0.369674	116135	116584	1534-1534	0.500000	57.0
rps15_p1	rps15	1	CCCCAGACGTCGTAGAGAAT	AAATAAGGAACTGCGTGGCA	20	20	55.0	45.0	58.6	58.1	10.5	0.0	0.705148	116585	116947	1401-1401	0.500000	54.4
rps15_p2	rps15	2	CCCCAGACGTCGTAGAGAAT	TGTCCAATGTACCTTGTGGC	20	20	55.0	50.0	58.6	58.1	10.5	9.6	0.705148	116585	116947	1167-1167	0.500000	54.4
rps15_p3	rps15	3	ATTCTATCCCCCAGACGTCG	AAATAAGGAACTGCGTGGCA	20	20	55.0	45.0	58.7	58.1	10.5	0.0	0.766458	116585	116947	1409-1409	0.500000	53.9
rps15_p4	rps15	4	ATTCTATCCCCCAGACGTCG	TGTCCAATGTACCTTGTGGC	20	20	55.0	50.0	58.7	58.1	10.5	9.6	0.766458	116585	116947	1175-1175	0.500000	53.9
rps15_p5	rps15	5	CCCCAGACGTCGTAGAGAAT	CCAATGTACCTTGTGGCTCA	20	20	55.0	50.0	58.6	57.8	10.5	9.6	0.808261	116585	116947	1164-1164	0.500000	53.5
rps15-ndhG_p1	rps15-ndhG	1	ATCACAAAGGGGTCTACGAA	AAATAAGGAACTGCGTGGCA	20	20	45.0	45.0	56.2	58.1	0.0	0.0	1.928377	116948	117053	519-519	0.500000	44.6
rps15-ndhG_p2	rps15-ndhG	2	TCACAAAGGGGTCTACGAAT	AAATAAGGAACTGCGTGGCA	20	20	45.0	45.0	56.2	58.1	0.0	0.0	1.928377	116948	117053	518-518	0.500000	44.6
rps15-ndhG_p3	rps15-ndhG	3	TGTGCATTAGGAAGAGTGGA	AAATAAGGAACTGCGTGGCA	20	20	45.0	45.0	56.2	58.1	0.0	0.0	1.936302	116948	117053	962-962	0.500000	44.5
rps15-ndhG_p4	rps15-ndhG	4	ATCACAAAGGGGTCTACGAA	CCGTTCATAAACAGCACAGC	20	20	45.0	50.0	56.2	57.8	0.0	0.0	2.079975	116948	117053	569-569	0.500000	43.4
rps15-ndhG_p5	rps15-ndhG	5	TCACAAAGGGGTCTACGAAT	CCGTTCATAAACAGCACAGC	20	20	45.0	50.0	56.2	57.8	0.0	0.0	2.079975	116948	117053	568-568	0.500000	43.4
ndhG_p1	ndhG	1	GCCACAAGGTACATTGGACA	CCTATCTCCAAAGTTCCGCA	20	20	50.0	50.0	58.1	57.3	22.9	0.0	0.787122	117054	117320	350-350	0.500000	53.7
ndhG_p2	ndhG	2	TGAGCCACAAGGTACATTGG	CCTATCTCCAAAGTTCCGCA	20	20	50.0	50.0	57.8	57.3	22.9	0.0	0.890236	117054	117320	353-353	0.500000	52.9
ndhG_p3	ndhG	3	AGCCACAAGGTACATTGGAC	CCTATCTCCAAAGTTCCGCA	20	20	50.0	50.0	57.8	57.3	22.9	0.0	0.891781	117054	117320	351-351	0.500000	52.9
ndhG_p4	ndhG	4	GCCACAAGGTACATTGGACA	TCCTATCTCCAAAGTTCCGC	20	20	50.0	50.0	58.1	57.0	22.9	0.0	1.077572	117054	117320	351-351	0.500000	51.4
ndhG_p5	ndhG	5	GCCACAAGGTACATTGGACA	CCTATCTCCAAAGTTCCGCAA	20	21	50.0	47.6	58.1	58.0	22.9	0.0	1.109817	117054	117320	350-350	0.500000	51.1
ndhG-psaC_p1	ndhG-psaC	1	GCCACAAGGTACATTGGACA	AGAGGTTTTCGGAGTCTCCA	20	20	50.0	50.0	58.1	58.0	22.9	19.2	0.106835	117321	118257	1929-1929	0.500000	59.1
ndhG-psaC_p2	ndhG-psaC	2	GCCACAAGGTACATTGGACA	TCCAGAGGTTTTCGGAGTCT	20	20	50.0	50.0	58.1	58.0	22.9	0.0	0.106835	117321	118257	1932-1932	0.500000	59.1
ndhG-psaC_p3	ndhG-psaC	3	TGCCACGCAGTTCCTTATTT	AGAGGTTTTCGGAGTCTCCA	20	20	45.0	50.0	58.1	58.0	0.0	19.2	0.106835	117321	118257	1695-1695	0.500000	59.1
ndhG-psaC_p4	ndhG-psaC	4	TGCCACGCAGTTCCTTATTT	TCCAGAGGTTTTCGGAGTCT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.106835	117321	118257	1698-1698	0.500000	59.1
ndhG-psaC_p5	ndhG-psaC	5	TGCCACGCAGTTCCTTATTT	TTGGACAAGCGGATTCACAA	20	20	45.0	45.0	58.1	58.0	0.0	0.0	0.121448	117321	118257	1214-1214	0.500000	59.0
psaC-ndhD_p1	psaC-ndhD	1	GTACCCAATGTGTTCGAGCT	ACTCATTCCTGGCAATGCAA	20	20	50.0	45.0	57.9	58.1	0.0	7.8	0.167275	118372	119267	1024-1024	0.500000	58.7
psaC-ndhD_p2	psaC-ndhD	2	GTACCCAATGTGTTCGAGCT	ACCACTCATTCCTGGCAATG	20	20	50.0	50.0	57.9	58.2	0.0	10.7	0.251394	118372	119267	1027-1027	0.500000	58.0
psaC-ndhD_p3	psaC-ndhD	3	TGTACCCAATGTGTTCGAGC	ACTCATTCCTGGCAATGCAA	20	20	50.0	45.0	58.2	58.1	0.0	7.8	0.269179	118372	119267	1025-1025	0.500000	57.8
psaC-ndhD_p4	psaC-ndhD	4	GTACCCAATGTGTTCGAGCT	TCATTCCTGGCAATGCAAGA	20	20	50.0	45.0	57.9	57.8	0.0	11.9	0.313843	118372	119267	1022-1022	0.500000	57.5
psaC-ndhD_p5	psaC-ndhD	5	TGTACCCAATGTGTTCGAGC	ACCACTCATTCCTGGCAATG	20	20	50.0	50.0	58.2	58.2	0.0	10.7	0.353298	118372	119267	1028-1028	0.500000	57.2
ndhD_p1	ndhD	1	AGACTCCGAAAACCTCTGGA	AGGCATTCTTTCTGGAGCAG	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.136895	119268	119498	1270-1270	0.500000	58.9
ndhD_p2	ndhD	2	TGGAGACTCCGAAAACCTCT	AGGCATTCTTTCTGGAGCAG	20	20	50.0	50.0	58.0	57.9	16.8	0.0	0.136895	119268	119498	1273-1273	0.500000	58.9
ndhD_p3	ndhD	3	AGACTCCGAAAACCTCTGGA	GGCATTCTTTCTGGAGCAGT	20	20	50.0	50.0	58.0	58.2	0.0	0.0	0.179542	119268	119498	1269-1269	0.500000	58.6
ndhD_p4	ndhD	4	TGGAGACTCCGAAAACCTCT	GGCATTCTTTCTGGAGCAGT	20	20	50.0	50.0	58.0	58.2	16.8	0.0	0.179542	119268	119498	1272-1272	0.500000	58.6
ndhD_p5	ndhD	5	AGACTCCGAAAACCTCTGGA	TCTGGAGCAGTATGGGCTAA	20	20	50.0	50.0	58.0	57.8	0.0	0.0	0.185808	119268	119498	1260-1260	0.500000	58.5
ndhD-ccsA_p1	ndhD-ccsA	1	AGACTCCGAAAACCTCTGGA	CACGCTGCTCTTAGGAACTT	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.097177	119499	120643	2231-2231	0.500000	59.2
ndhD-ccsA_p2	ndhD-ccsA	2	TGGAGACTCCGAAAACCTCT	CACGCTGCTCTTAGGAACTT	20	20	50.0	50.0	58.0	57.9	16.8	0.0	0.097177	119499	120643	2234-2234	0.500000	59.2
ndhD-ccsA_p3	ndhD-ccsA	3	AGACTCCGAAAACCTCTGGA	TAGACACGCTGCTCTTAGGA	20	20	50.0	50.0	58.0	57.9	0.0	0.0	0.135400	119499	120643	2235-2235	0.500000	58.9
ndhD-ccsA_p4	ndhD-ccsA	4	TGGAGACTCCGAAAACCTCT	TAGACACGCTGCTCTTAGGA	20	20	50.0	50.0	58.0	57.9	16.8	0.0	0.135400	119499	120643	2238-2238	0.500000	58.9
ndhD-ccsA_p5	ndhD-ccsA	5	TTGCATTGCCAGGAATGAGT	CACGCTGCTCTTAGGAACTT	20	20	45.0	50.0	58.1	57.9	15.1	0.0	0.158567	119499	120643	1834-1834	0.500000	58.7
ccsA_p1	ccsA	1	TGGCGAAACCTTGGGTAAAA	CACGCTGCTCTTAGGAACTT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.154889	120644	120919	580-580	0.500000	58.8
ccsA_p2	ccsA	2	GCGGGTTGATTTGCATTTCA	CACGCTGCTCTTAGGAACTT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.163467	120644	120919	616-616	0.500000	58.7
ccsA_p3	ccsA	3	TGCGGGTTGATTTGCATTTC	CACGCTGCTCTTAGGAACTT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.163467	120644	120919	617-617	0.500000	58.7
ccsA_p4	ccsA	4	TGGCGAAACCTTGGGTAAAA	AGACTCCGACTGAGAACGAA	20	20	45.0	50.0	57.9	58.1	0.0	0.4	0.175290	120644	120919	881-881	0.500000	58.6
ccsA_p5	ccsA	5	GCGGGTTGATTTGCATTTCA	AGACTCCGACTGAGAACGAA	20	20	45.0	50.0	57.9	58.1	0.0	0.4	0.183868	120644	120919	917-917	0.500000	58.5
ccsA-trnL-UAG_p1	ccsA-trnL-UAG	1	TGGCGAAACCTTGGGTAAAA	CACGCTGCTCTTAGGAACTT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.154889	120920	121069	580-580	0.500000	58.8
ccsA-trnL-UAG_p2	ccsA-trnL-UAG	2	GCGGGTTGATTTGCATTTCA	CACGCTGCTCTTAGGAACTT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.163467	120920	121069	616-616	0.500000	58.7
ccsA-trnL-UAG_p3	ccsA-trnL-UAG	3	TGCGGGTTGATTTGCATTTC	CACGCTGCTCTTAGGAACTT	20	20	45.0	50.0	57.9	57.9	0.0	0.0	0.163467	120920	121069	617-617	0.500000	58.7
ccsA-trnL-UAG_p4	ccsA-trnL-UAG	4	TGGCGAAACCTTGGGTAAAA	AGACTCCGACTGAGAACGAA	20	20	45.0	50.0	57.9	58.1	0.0	0.4	0.175290	120920	121069	881-881	0.500000	58.6
ccsA-trnL-UAG_p5	ccsA-trnL-UAG	5	GCGGGTTGATTTGCATTTCA	AGACTCCGACTGAGAACGAA	20	20	45.0	50.0	57.9	58.1	0.0	0.4	0.183868	120920	121069	917-917	0.500000	58.5
trnL-UAG-trnN-GUU_p1	trnL-UAG-trnN-GUU	1	TGGCGAAACCTTGGGTAAAA	GGCAGAATAGCAGAGCAAGT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.104364	121165	122732	2565-2565	0.500000	59.2
trnL-UAG-trnN-GUU_p2	trnL-UAG-trnN-GUU	2	GCGGGTTGATTTGCATTTCA	GGCAGAATAGCAGAGCAAGT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.112943	121165	122732	2601-2601	0.500000	59.1
trnL-UAG-trnN-GUU_p3	trnL-UAG-trnN-GUU	3	TGCGGGTTGATTTGCATTTC	GGCAGAATAGCAGAGCAAGT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.112943	121165	122732	2602-2602	0.500000	59.1
trnL-UAG-trnN-GUU_p4	trnL-UAG-trnN-GUU	4	AAGTTCCTAAGAGCAGCGTG	GGCAGAATAGCAGAGCAAGT	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.121584	121165	122732	2005-2005	0.500000	59.0
trnL-UAG-trnN-GUU_p5	trnL-UAG-trnN-GUU	5	TGGCGAAACCTTGGGTAAAA	AAGCGAGCCCTTTCATTCAA	20	20	45.0	45.0	57.9	58.1	0.0	0.0	0.159134	121165	122732	2288-2288	0.500000	58.7