Hotspot

ycf1

SSC · 118689-122597 · 3909 bp

Back to Polystichum

Pi 0.24

MarkerSeek score 79.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 3909
variable_sites: 1746
pi: 0.24
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCAACTAGCCACGGTATCAA	TGAACTTGTCGCGTTACACA	50.0	45.0	58.0	58.1	945-948 bp	0.75	77.5
ycf1_p2	GCAACTAGCCACGGTATCAA	TTTGGTGGCATCAATTTCGC	50.0	45.0	58.0	57.9	1005 bp	0.5	65.4
ycf1_p3	GCAACTAGCCACGGTATCAA	ATCAGCCTGAGCAATCTTCG	50.0	50.0	58.0	58.0	685 bp	0.25	49.5
ycf1_p4	GCAACTAGCCACGGTATCAA	CCTTTCGCTGGGTATGACTC	50.0	55.0	58.0	58.1	641 bp	0.25	49.4
ycf1_p5	AGAGGCCAAGTTTCGTTGTT	ATCAGCCTGAGCAATCTTCG	45.0	50.0	57.9	58.0	1029 bp	0.25	49.2