Hotspot

ndhH

SSC · 116834-118012 · 1179 bp

Back to Polystichum

Pi 0.222

MarkerSeek score 84.30

Primer available yes

Label ndhH

Feature Metadata

label_name: ndhH
feature_type: gene
region: SSC
length_bp: 1179
variable_sites: 510
pi: 0.222
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhH_p1	CCCAGTGGACCTGCATATTC	CCATCTGGAATCACACTCCG	55.0	55.0	58.0	58.1	1662 bp	0.75	73.3
ndhH_p2	ACACCTTTCGTTCAAGCCAT	CCATCTGGAATCACACTCCG	45.0	55.0	58.0	58.1	1613 bp	0.5	61.7
ndhH_p3	ACACCTTTCGTTCAAGCCAT	GGGGGCAAAGGGATATGAAG	45.0	55.0	58.0	58.0	2024 bp	0.25	49.8
ndhH_p4	CCCAGTGGACCTGCATATTC	GGGGGCAAAGGGATATGAAG	55.0	55.0	58.0	58.0	2073 bp	0.25	49.8
ndhH_p5	CTCGGATATGCATCTACGCC	GGGGGCAAAGGGATATGAAG	55.0	55.0	58.1	58.0	2593 bp	0.25	49.3