Hotspot

ndhE

SSC · 112958-113260 · 303 bp

Back to Polystichum

Pi 0.249

MarkerSeek score 76.40

Primer available yes

Label ndhE

Feature Metadata

label_name: ndhE
feature_type: gene
region: SSC
length_bp: 303
variable_sites: 148
pi: 0.249
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE_p1	CACTGCGTACAACCGATACA	GTACTTCACCGCTTCAGGAG	50.0	55.0	58.0	58.0	1075-1080 bp	0.5	63.6
ndhE_p2	CACTGCGTACAACCGATACA	CTCTTCGGGGTTGGTTTTCA	50.0	50.0	58.0	58.0	1249-1254 bp	0.5	63.1
ndhE_p3	CACTGCGTACAACCGATACA	GGAAGAGAAAAGAGAGGGCG	50.0	55.0	58.0	58.0	1427 bp	0.25	49.7
ndhE_p4	CACTGCGTACAACCGATACA	AGAAAAGAGAGGGCGCAAAA	50.0	45.0	58.0	58.0	1422 bp	0.25	49.7
ndhE_p5	CACTGCGTACAACCGATACA	TCCGTTCGAGCTTCTTTCAG	50.0	50.0	58.0	57.9	901 bp	0.25	49.4