Hotspot

ndhD

SSC · 110736-112244 · 1509 bp

Back to Polystichum

Pi 0.228

MarkerSeek score 83.40

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1509
variable_sites: 660
pi: 0.228
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TTGGGAGTCAACTTGTTGGG	TGTATCGGTTGTACGCAGTG	50.0	50.0	57.9	58.0	2299-2319 bp	0.5	64.9
ndhD_p2	GGCTGATGTAGAAGCAACGA	TGTATCGGTTGTACGCAGTG	50.0	50.0	58.0	58.0	2276 bp	0.25	49.8
ndhD_p3	AGTGGGAAGGGGGTAGTTAC	TGTATCGGTTGTACGCAGTG	55.0	50.0	58.0	58.0	2176 bp	0.25	49.6
ndhD_p4	GGCTGATGTAGAAGCAACGA	TCAAAGCTTCGAGATTGCGA	50.0	45.0	58.0	57.9	2016 bp	0.25	49.3
ndhD_p5	ATTTCAATTTGTGCCAGCGG	TGTATCGGTTGTACGCAGTG	45.0	50.0	57.9	58.0	2070 bp	0.25	49.2