Hotspot

ndhA

SSC · 114760-116832 · 2073 bp

Back to Polystichum

Pi 0.234

MarkerSeek score 82.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2073
variable_sites: 920
pi: 0.234
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	TCGGACACATACTTCGCAAG	AGGTTTCACCAGCAGACAAG	50.0	50.0	58.0	58.0	2972-3000 bp	0.5	63.9
ndhA_p2	CTCGGACACATACTTCGCAA	GGTTTCACCAGCAGACAAGA	50.0	50.0	58.0	58.0	2972-3000 bp	0.5	63.9
ndhA_p3	TCGGACACATACTTCGCAAG	GGTTTCACCAGCAGACAAGA	50.0	50.0	58.0	58.0	2971-2999 bp	0.5	63.9
ndhA_p4	CTCGGACACATACTTCGCAA	TCAGTTGGGGCTTATCTGGA	50.0	50.0	58.0	58.0	2975 bp	0.25	49.6
ndhA_p5	TCGGACACATACTTCGCAAG	TCAGTTGGGGCTTATCTGGA	50.0	50.0	58.0	58.0	2974 bp	0.25	49.6