Hotspot

ccsA

SSC · 109393-110337 · 945 bp

Back to Polystichum

Pi 0.221

MarkerSeek score 82.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 945
variable_sites: 398
pi: 0.221
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GATTCGAACCTGTGACCTCC	CAGGAATGAGCGGTTTCGTA	55.0	50.0	58.0	58.0	2366-2404 bp	0.5	67.0
ccsA_p2	GGATTCGAACCTGTGACCTC	CAGGAATGAGCGGTTTCGTA	55.0	50.0	58.0	58.0	2367-2405 bp	0.5	67.0
ccsA_p3	CTCTTAGGAAGCAGTGCCAG	CAGGAATGAGCGGTTTCGTA	55.0	50.0	58.0	58.0	2010-2030 bp	0.5	66.3
ccsA_p4	CTTAGGAAGCAGTGCCAGAG	CAGGAATGAGCGGTTTCGTA	55.0	50.0	58.0	58.0	2008-2028 bp	0.5	66.3
ccsA_p5	GATTCGAACCTGTGACCTCC	GTAACTACCCCCTTCCCACT	55.0	55.0	58.0	58.0	1774 bp	0.25	49.6