Hotspot

chlN-trnN-GUU

SSC · 125556-126950 · 1395 bp

Back to Polystichum

Pi 0.248

MarkerSeek score 84.10

Primer available yes

Label chlN-trnN-GUU

Feature Metadata

label_name: chlN-trnN-GUU
feature_type: igs
region: SSC
length_bp: 1395
variable_sites: 666
pi: 0.248
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN-trnN-GUU_p1	ACAGACTGACGCTTCGATTC	GTCACTGGATCAAACGACGA	50.0	50.0	58.0	57.9	2433-2724 bp	0.75	79.0
chlN-trnN-GUU_p2	ACAGACTGACGCTTCGATTC	CCCCCACGAACCTTTCATAG	50.0	55.0	58.0	58.0	2349 bp	0.25	49.6
chlN-trnN-GUU_p3	GAACAGACTGACGCTTCGAT	CCCCCACGAACCTTTCATAG	50.0	55.0	58.0	58.0	2351 bp	0.25	49.6
chlN-trnN-GUU_p4	CCAACCCGATACAGGAACAG	CCCCCACGAACCTTTCATAG	55.0	55.0	58.0	58.0	2365 bp	0.25	49.5
chlN-trnN-GUU_p5	GATTCCGCCATTGCATAACG	CCCCCACGAACCTTTCATAG	50.0	55.0	58.0	58.0	1893 bp	0.25	49.4