Hotspot

ycf1-chlN

SSC · 122598-124169 · 1572 bp

Back to Polystichum

Pi 0.229

MarkerSeek score 82.90

Primer available yes

Label ycf1-chlN

Feature Metadata

label_name: ycf1-chlN
feature_type: igs
region: SSC
length_bp: 1572
variable_sites: 677
pi: 0.229
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-chlN_p1	ATAGTGCTCGATCTTCGGGA	GAATCGAAGCGTCAGTCTGT	50.0	50.0	58.0	58.0	2690-2698 bp	0.75	76.8
ycf1-chlN_p2	TAGTGCTCGATCTTCGGGAT	GAATCGAAGCGTCAGTCTGT	50.0	50.0	58.0	58.0	2689-2697 bp	0.75	76.8
ycf1-chlN_p3	ATAGTGCTCGATCTTCGGGA	ATCGAAGCGTCAGTCTGTTC	50.0	50.0	58.0	58.0	2688-2696 bp	0.5	64.5
ycf1-chlN_p4	TAGTGCTCGATCTTCGGGAT	ATCGAAGCGTCAGTCTGTTC	50.0	50.0	58.0	58.0	2687-2695 bp	0.5	64.5
ycf1-chlN_p5	GCGTACCCTTTTCTTCGGAT	ATCGAAGCGTCAGTCTGTTC	50.0	50.0	58.0	58.0	2812 bp	0.25	49.7