Hotspot

trnN-GUU-rpl32

SSC · 104302-107846 · 3545 bp

Back to Polystichum

Pi 0.205

MarkerSeek score 79.50

Primer available yes

Label trnN-GUU-rpl32

Feature Metadata

label_name: trnN-GUU-rpl32
feature_type: igs
region: SSC
length_bp: 3545
variable_sites: 1367
pi: 0.205
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-rpl32_p1	ACGAAAGTAGCAGCTTACGG	CCGCTATCCTGTTTCTGGTC	50.0	55.0	58.0	58.1	1021-1028 bp	0.75	77.5
trnN-GUU-rpl32_p2	ACGAAAGTAGCAGCTTACGG	GTATACCCCCGCTATCCTGT	50.0	55.0	58.0	58.1	1029-1036 bp	0.75	77.4
trnN-GUU-rpl32_p3	ACGAAAGTAGCAGCTTACGG	ACTCCACGATGTCCCAGTTA	50.0	50.0	58.0	58.1	1287-1294 bp	0.75	77.4
trnN-GUU-rpl32_p4	ACGAAAGTAGCAGCTTACGG	TACTCCACGATGTCCCAGTT	50.0	50.0	58.0	58.1	1288-1295 bp	0.75	77.4
trnN-GUU-rpl32_p5	ACGAAAGTAGCAGCTTACGG	CGCTATCCTGTTTCTGGTCC	50.0	55.0	58.0	58.1	1020 bp	0.5	66.4