Hotspot

trnT-GGU-rps14

LSC · 35150-35823 · 674 bp

Back to Polystichum

Pi 0.0692
MarkerSeek score 84.80
Primer available yes
Label trnT-GGU-rps14

Feature Metadata

label_name
trnT-GGU-rps14
feature_type
igs
region
LSC
length_bp
674
variable_sites
85
pi
0.0692
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-rps14_p1 CTCTACCGCTGAGTTAAGGG GAGTATCCAAATCAAGTTGGT 55.0 38.1 57.1 53.5 715-772 bp 1.0 50.4
trnT-GGU-rps14_p2 GGATTTGAACCGACGGCTTA GAGTATCCAAATCAAGTTGGT 50.0 38.1 58.3 53.5 753-810 bp 1.0 50.3
trnT-GGU-rps14_p3 GGATTTGAACCGACGGCTTA AGTATCCAAATCAAGTTGGT 50.0 35.0 58.3 52.0 752-809 bp 1.0 50.3
trnT-GGU-rps14_p4 GATTTGAACCGACGGCTTAC GAGTATCCAAATCAAGTTGGT 50.0 38.1 57.2 53.5 752-809 bp 1.0 50.3
trnT-GGU-rps14_p5 CCCCTTGTCGGATTTGAACC GAGTATCCAAATCAAGTTGGT 55.0 38.1 58.8 53.5 762-819 bp 1.0 50.3